General Cell Based Assays
Eurofins Discoverx PathHunter eXpress MCHR2 U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0691E3CP5L)
The PathHunter MCHR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. MCH) based activation of MCHR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MCHR2 and EA-tagged β-Arrestin-2. Activation of MCHR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Sigma Aldrich Fine Chemicals Biosciences Bilirubin Assay Kit suffic
Bilirubin also known as hematoidin is a degradation product formed as a result of heme catabolism in the liver. Bilirubin circulates in the blood stream as either the unconjugated insoluble form (indirect bilirubin) or the soluble glucuronide-conjugated form (direct bilirubin). Conjugated bilirubin moves from the bile canaliculi of the liver to the gall bladder where it is excreted into the small intestine during digestion. High levels of bilirubin can result in jaundice and may indicate liver disease blood disorders or blockage of the bile ducts.
Eurofins Discoverx PathHunter Human BCMA Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human BCMA Stable Cell Line Internalization Assay (U2OS) measures internalization of the BCMA receptor via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress MRGPRE CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0438E2ACP1L)
The PathHunter MRGPRE eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of MRGPRE GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MRGPRE and EA-tagged β-Arrestin-2. Activation of MRGPRE stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human GPR173 β-Arrestin Cell-Based Assay Kit (HEK 293)
The PathHunter Human GPR173 β-Arrestin Stable Cell Line Assay (HEK 293) contains a stable clonal cell line used to measure GPR173 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human GPR135 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR135 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR135 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx cAMP Hunter eXpress PTGER2 DLD1 GPCR Assay, 10-Plate Kit. (95-0176E13CP25L)
The cAMP Hunter PTGER2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prostaglandin E2) based activation of PTGER2 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PTGER2, a naturally Gs coupled GPCR. Upon activation of PTGER2, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
TRIBIOSCIENCE INC EGFR Kinase Activity Assay 100 Tests
Tribioscience’s Fast EGFR Kinase Activity Assay is designed to quantitatively detect EGFR Kinase Activity levels in different tissues including skin, muscle, neural, serum, and other biological samples. In this assay EGFR phosphorates tyrosine-based substrate in the presence of co-substrate ATP. The phosphotyrosine concentration can be detected with anti-phorsphotyrosine based sandwich ELISA technology. The assay combines multiple reactions into a one-step. The measurement can be finished in 1.5 hours. The detection range is from 78 to 5000 pg/mL. The levels of sample EGFR Kinase activity are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural EGFR Kinase Activity.
