General Cell Based Assays
Sigma Aldrich Fine Chemicals Biosciences Direct DetectR Assay free
Quantity OptionsDDAC00010-GR 50 CardsDDAC00010-4P 200 CardsDDAC00010-8P 400 CardsThe Direct Detect Assay-free Cards are desgined for use in the the Direct Detect Spectrometer. The card contains four hydrophilic polytetrafluoroethylene (PTfE) membrane positions sized for easy sample application and surrounded by a hydrophobic ring to retain analyzed sample within the IR beam. A 2 muL sample volume is recommended in order to assure proper sample application drying and measurement.The Assay-free card can be inserted into the system and analyzed immediately after preparation. The Direct Detect spectrometer has a built in drying system that will automatically dry the sample to the point where it will give an accurate reading. The Direct Detect Assay-free card is intended for a one-time protein application however already prepared cards can be stored and reread later.
Cayman Chemical BIlIrubIn ConjugatesodIM500mg
A stable, water-soluble ditaurate derivative of bilirubin meant to mimic endogenous bilirubin glucuronide derivatives; exhibits anti-oxidant and antimutagenic effects
Sigma Aldrich Fine Chemicals Biosciences IDH Activity Assay Kit suf
Isocitrate dehydrogenase (IDH) catalyzes the conversion of isocitrate to alpha-ketoglutarate. In eukaryotes there are three isozymes of IDH the mitochondrial IDH2 and IDH3 and the cytoplasmic/ peroxisomal IDH1. All three IDH family members require the presence of a divalent cation (Mg2 or Mn2) and either the electron-accepting cofactor NADP (IDH1 and IDH2) or NAD (IDH3) for their enzymatic activity. IDH1 and IDH2 mutations resulting in neomorphic enzymatic activity are found in certain cancers such as glioblastoma acute myeloid leukemia and colon cancer. This neoactivity shows a change in the substrate specificity resulting in the conversion of alpha-ketoglutarate to 2-hydroxyglutarate. Mutations in IDH family members are also associated with Ollier disease and Maffucci syndrome.
FUJIFILM BIOSCIENCES INC ADRA Kit
Amino acid Derivative Reactivity Assay ADRA is an alternative method of skin sensitization test to animal testing ADRA significantly reduces the number of chemical substances used as a reaction reagent by using a novel amino acid derivative having a naphthalene ring capable of high sensitivity detection by ultraviolet rays Also skin sensitization tests are possible even at about 1/100 by conventional methods It is possible to evaluate skin sensitization with high accuracy without causing chemical substances to precipitate in the reaction solution It evaluates whether a chemical substance provokes an allergic reaction in the skin without using animal models Reagent kit for performing ADRA as described in OECD TG 442C
Bioassay Systems QuantiChrom™ Acetylcholinesterase Assay Kit. For quantitative determination of acetylcholinesterase activity and evaluation of acetylcholinesterase inhibitors.
For quantitative determination of acetylcholinesterase activity and evaluation of acetylcholinesterase inhibitors. Key Features: Sensitive and accurate. Detection range 10 to 600 U/L AChE activity in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at 2 min and 10 min at room temperature. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD412nm. Samples: Blood, serum, plasma etc. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 10 U/L.
Cell Biolabs Inc CytoSelect™ 24-Well Cell Migration and Invasion Assay Combo Kit (8 µm, Fluorometric), 2x12 assays
Chemotaxis, a type of cell migration, is a highly integrated, multistep process that orchestrates embryonic morphogenesis, tissue repair and regeneration. Cell invasion is the ability of malignant tumor cells to invade normal surrounding tissue, which contributes in large part to the significant morbidity and mortality of cancers. The CytoSelect™ Cell Migration and Invasion Assay Kit utilizes polycarbonate membrane inserts (8 µm pore size) to assay the invasive properties of cells. The kit contains twelve 8 um uncoated polycarbonate membrane inserts, and another twelve 8 um polycarbonate membrane inserts coated with an ECM gel layer in a 24-well plate. Cells must degrade the ECM layer and then move through the pores of the membrane. Invasive cells are then quantified using the provided fluorometric dye.
Bioassay Systems EnzyChrom™ Aconitase Assay Kit. For quantitative determination of aconitase enzyme activity and high-throughput screening assays for aconitase modulator.
For quantitative determination of aconitase enzyme activity and high-throughput screening assays for aconitase modulator. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.5 to 100 U/L for 20 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handlingsystems for processing thousands of samples per day. Method: OD565nm. Samples: Biological samples (e.g. cell lysate, tissue homogenate, serum, etc.). Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.5 U/L.
FUJIFILM BIOSCIENCES INC LumiMAT Pyrogen Detection Kit
MAT NOMO-1 cell line for the detection of endotoxin and non-endotoxin pyrogens
Bioassay Systems QuantiChrom™ Creatinine Assay Kit. For quantitative determination of creatinine and evaluation of drug effects on creatinine metabolism.
For quantitative determination of creatinine and evaluation of drug effects on creatinine metabolism. Key Features: Sensitive and accurate. Use 30 µL samples. Detection limit 0.10 mg/dL (8 µM) creatinine in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Assays can be executed in 96-well plate or cuvet. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples. Method: OD510nm. Samples: Urine, serum, plasma and biological preparations. Species: All. Procedure: Assay takes 20 min. Kit size: 500 tests. Detection limit: 0.1 mg/dL (8 µM).